ABSTRACT
Giardia lamblia is one of the most common diarrhea-related parasites in humans, where infection ranges from asymptomatic to acute or chronic disease. Because of the non-characteristic symptoms of giardiasis, as well as significant prevalence of cyst carriers, it is necessary to upgrade parasitological techniques for examination of feces, in order to avoid false negative results. Accurate diagnosis is important to exclude other parasitic causes of diarrhea. The present study is designed to assess the efficacy of flow cytometry [FC] as a sensitive method for detection of G. lamblia cysts in stool samples in comparison with other standard conventional diagnostic methods. 70 patients [30 males and 40 females] ranging in age from 5 to 60 years were included in this study. Stool samples were taken from each patient on three successive days to detect Giardia cysts and evaluate the intensity of infection. Different methods were used that included concentration methods, permanent stained slides, immunofluorescent antibody test [IFAT] and FC. Giardia lamblia cysts were detected in 24 samples by IFAT with detection rate of 34.3%. Using trichrome stain 20 samples [28.6%] were positive, modified Ziehl- Neelsen Stain [MZN] detected 11samples [15.7%], while Acridine Orange [AO] detected 14 positive samples [20%]. Flow cytometry detected 18 samples with detection rate of 25.7%. Sensitivity and specificity of FC were 90% and 100%, respectively and IFAT sensitivity and specificity were100% and 92%, respectively. Results of the present study clearly demonstrated that incorporation of IFAT and FC can improve sensitivity of detection of Giardia cysts in stool samples. Although FC is more expensive than the other staining methods and IFAT, it is rapid, simple and accurate in estimating the quantity of parasites in each sample. Thus, FC can be recommended for detection of protozoa in stool
Subject(s)
Humans , Male , Female , Flow Cytometry/statistics & numerical data , Feces/parasitology , Fluorescent Antibody Technique, Direct/statistics & numerical data , Azo Compounds/adverse effects , Acridine Orange/adverse effectsABSTRACT
El VIH=SIDA es un importante problema de Salud Publica por la cantidad de personas que han fallecido por esta causa en todoel mundo 40 millones de infectados hasta fines del año 2001(11). Uno de los propocitos mas importantes de la investigacion medica, es sin duda conocer los transtornos que subyacen detras dela clinica de la infeccion por el virus de la inmunodeficiencia humana que posee una serie de peciulariedades, algunas conocidas y otras por descubrir> Hasta el momento no se ha podido encontrar un tratamiento eficaz y son los estudios actuales en este campo que tratan de encontrarlo. PREGUNTA DE INVESTIGACION: Cual es el comportamiento cuantitativo de las poblaciones linfositarias CD16+ y CD56+ en pacientes VIH(+) durante la evolucion dela infeccion?
Subject(s)
Humans , Male , Female , /administration & dosage , Fluorescent Antibody Technique, Direct/classification , Fluorescent Antibody Technique, Direct/statistics & numerical data , Fluorescent Antibody Technique, Direct/standards , Acquired Immunodeficiency Syndrome/diagnosisABSTRACT
A seroprevalência da infecçäo pelo vírus herpes humano 6(VHH-6) foi investigada em 526 brasileiros e 250 japoneses e descendentes residentes no Recife e regiöes vizinhas no Nordeste do brasil. Um total de 776 soros de indivíduos foram examinados para pesquisa de anticorpos IgG pela técnica de imunofluorescência anti-complemento. O percentual de soropositividade entre os dois grupos foi muito próximo: 73,3por cento para os brasileiros oriundos da regiäo e 77,2 por cento para os de ascendência Japonesa. Nas duas populaçöes estudadas as mulheres mostraram índices de positividade discretamente mais altos que os homens. Estes índices foram significativamente mais altos em indivíduos considerados de nível sócio econômico mais baixo. A alta prevalência de anticorpos anti-VHH-6 entre crianças indica que a infecçäo por este vírus ocorre muito cedo nesta regiäo. Análise comparativa mostra näo haver associaçäo entre infecçäo pelo citomegavirus (CMV) e VHH-6